Journal: Nature Communications

Article Title: Dextran-based T-cell expansion nanoparticles for manufacturing CAR T cells with augmented efficacy

doi: 10.1038/s41467-025-67868-1

Figure Lengend Snippet: a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or naked NPs both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.

Article Snippet: White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 μm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between DynabeadsTM and T-Expand.

Techniques: Imaging, Incubation, Activation Assay, Two Tailed Test

Journal: Science Advances

Article Title: A robust adhesive microneedle for oral infections therapy via synergistic antibacterial and neutrophil-macrophage axis immunomodulation

doi: 10.1126/sciadv.aee4401

Figure Lengend Snippet: ( A ) 3D-FEA stress distribution maps of the peel adhesion behavior between the PCA@FeCO flat/MN patch and the mucosal tissue. ( B ) The peel, tensile, and shear adhesive strengths of PCA@FeCO flat and PCA@FeCO MN. Data are presented as means ± SD ( n = 4). ( C ) Schematic illustration of MN taper. ( D ) Stress distribution maps of the mucosal tissue from 3D-FEA of the peel adhesion behavior between PCA@FeCO MNs with different tapers and the mucosa. ( E ) Peak stress in the mucosa induced by PCA@FeCO MN with different tapers in the 3D-FEA model. ( F ) Peak stress of PCA@FeCO MN with different tapers in the 3D-FEA model. ( G ) Compression performance test of PCA@FeCO MN with different tapers. ( H ) Morphology and composition of 30°-PCA@FeCO MNs: Photograph, magnified view, SEM image, and EDS analysis. Among them, photograph and magnified view are the same as those in fig. S4. ( I ) Cross-sectional fluorescence images demonstrating the distribution of rhodamine B–labeled PCA@FeCO MN in mucosa after 10 min. ( J ) Robust adhesion of PCA@FeCO MN to the buccal mucosa, palatal mucosa, gingiva, and tongue of a rat.

Article Snippet: A 3D-FEA simulation was conducted in the ABAQUS software.

Techniques: Shear, Adhesive, Fluorescence, Labeling